Optimization and Kinetic Modelling of Total Phenols and Flavonoids Extraction from Tilia cordata M. Flowers

ABSTRACT The main goal was to optimize and establish the kinetics thermodynamics of extraction process. An investigation into effects ethanol concentration (0-100 %), liquid solid ratio (30-60 mL g-1), temperature (25-65 °C), time (40-200 min) on total phenols (TP) flavonoids (TF) from linden (Tilia cordata M.) flowers performed using single-factor experiments. Experimental results showed that conditions had a significant effect phenolic compounds. optimized were 50 % ethanol, 60 g-1 for 120 min at 55 °C, with values 17.05 mg GAE dry weight (d.w.) TP 13.32 CE d.w. TF. Extracted compounds analyzed by HPLC. antioxidant activity extracts evaluated DPPH ABTS assays. applicability different mathematical models (model based unsteady diffusion through plant material, model film theory Ponomaryov) describe process TF studied as well. applied good agreement experimental results, which allows their application in modelling optimizing solid/liquid extract flowers. Keywords: Extraction conditions, compounds, activity, models.